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Therapy of genomic DNA with bisulfite and subsequent PCR in the region of desire delivers PCR items through which initially unmethylated cytosines happen as thymines and methylated cytosines as cytosines. Subcloning and sequencing of your PCR goods
Treatment of PCR products and solutions with exonuclease I and heat-labile alkaline phosphatase improves the visibility of blended bisulfite restriction Evaluation
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As a way to ameliorate the efficiency of PCR, we developed a fresh primer-layout program named BiSearch, accessible within the Internet. It has the special house of analyzing the primer pairs for mispriming web sites within the bisulfite-taken care of genome and decides likely non-distinct amplification solutions that has a new research algorithm. The options of primer-design and style and Evaluation for mispriming web sites can be used sequentially or sep...
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